Mechanism of lignin inhibition of enzymatic biomass deconstruction

Mechanism of lignin inhibition of enzymatic biomass deconstruction

BACKGROUND The conversion of plant biomass to ethanol via enzymatic cellulose hydrolysis offers a potentially sustainable route to biofuel production. However, the inhibition of enzymatic activity in pretreated biomass by lignin severely limits the efficiency of this process. RESULTS By performing atomic-detail molecular dynamics simulation of a biomass model containing cellulose, lignin, and...

Supplementing with Non-Glycoside Hydrolase Proteins Enhances Enzymatic Deconstruction of Plant Biomass

The glycoside hydrolases (GH) of Caldicellulosiruptor bescii are thermophilic enzymes, and therefore they can hydrolyze plant cell wall polysaccharides at high temperatures. Analyses of two C. bescii glycoside hydrolases, CbCelA-TM1 and CbXyn10A with cellulase and endoxylanase activity, respectively, demonstrated that each enzyme is highly thermostable under static incubation at 70°C. Both enzy...

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release.

The conversion of lignocellulosic biomass to fuels, chemicals, and other commodities has been explored as one possible pathway toward reductions in the use of non-renewable energy sources. In order to identify which plants, out of a diverse pool, have the desired chemical traits for downstream applications, attributes, such as cellulose and lignin content, or monomeric sugar release following a...

Enzymatic Hydrolysis of Biomass: Effects of Crystallinity, Particle Size, and Lignin Removal

Enzymatic hydrolysis of cellulose in plant and wood cell walls is expected to be affected by its chemical composition as well as structural and morphological features. In the present study, different crystallinity cellulose samples and varying size fractionated loblolly pine wood cell wall particles were hydrolyzed using a mixture of Celluclast and Novozyme 188. In pure cellulose samples of Wha...

Mechanism of the inhibition of enzymatic halogenation by antithyroid agents.